Manipulation and analysis of cell cycle-dependent processes in budding yeast

Michael G. Stewart*, Talia C. Scheel*, Ahmed A. Abouelghar*, Sara E. Hoppe*, Matthew P. Miller

J. Vis. Exp. (223), e68887

Stu2 performs an essential kinetochore function independent of its microtubule polymerase activity

Ahmed A. Abouelghar*, Joseph S. Carrier*, Julia R. Torvi, Erin Jenson, Chloe Jones, Binnu Gangadharan, Elisabeth G. Prinslow, Luke M. Rice, Brent Lagesse, Georjana Barnes, Matthew P. Miller

J Cell Biol (2025) 224 (10): e202209025

A coordinated kinase and phosphatase network regulates Stu2 recruitment to yeast kinetochores

Michael G. Stewart, Joseph S. Carrier, Jacob A. Zahm, Stephen C. Harrison, Matthew P. Miller

J Cell Biol (2025) 224 (8): e202410196

A conserved site on Ndc80 complex facilitates dynamic recruitment of Mps1 to yeast kinetochores to promote accurate chromosome segregation

Emily J. Parnell, Erin E. Jenson, Matthew P. Miller

Curr Bio 2024 May 21;34(11):P2294-2307.E4

Structural Basis of Stu2 Recruitment to Yeast Kinetochores

Jacob A. Zahm, Michael G. Stewart, Joseph S. Carrier, Stephen C. Harrison, Matthew P. Miller

eLife 2021;10:e65389

Cdk1 Phosphorylation of the Dam1 Complex Strengthens Kinetochore-Microtubule Attachments

Abraham Gutierrez 1, Jae Ook Kim 2, Neil T Umbreit 2, Charles L Asbury 3, Trisha N Davis 2, Matthew P Miller 4, Sue Biggins 5

Curr Bio 2020 Nov 16;30(22):4491-4499.e5

chTOG is a conserved mitotic error correction factor

Jacob A Herman, Matthew P Miller, Sue Biggins

eLife 2020 Dec 30;9:e61773

A Stu2-mediated intrinsic tension-sensing pathway promotes chromosome biorientation in vivo

Miller, M. P., Evans, R. K., Zelter, A., Geyer, E. A., MacCoss, M., Rice, L. M., Davis, T. N., Asbury, C. L., Biggins, S.

PLoS Genet 2019 Oct 4;15(10):e1008423

Design principles of a microtubule polymerase

Geyer, E. A., Miller, M. P., Brautigam, C. A, Biggins, S., Rice, L. M., 

eLife 2018 Jun 13;7. pii: e34574.

A TOG Protein Confers Tension Sensitivity to Kinetochore-Microtubule Attachments

Miller, M. P., Asbury, C. L. & Biggins, S.

Cell 165(6):1428-1439 (2016)

Kinetochores require oligomerization of Dam1 complex to maintain microtubule attachments against tension and promote biorientation

Umbreit, N. T., Miller, M. P., et al.

Nat. Commun. 5, 4951 (2014)

Meiosis I: when chromosomes undergo extreme makeover

Miller, M. P., Amon, A. & Unal, E. 

Curr. Opin. Cell Biol. 25(6):687-96 (2013)

Polo kinase Cdc5 is a central regulator of meiosis I

Attner, M. A., Miller, M. P., Ee, L.-S., Elkin, S. K. & Amon, A.

Proc. Natl. Acad. Sci. U. S. A. 110(35):14278-83 (2013)

Meiosis I chromosome segregation is established through regulation of microtubule-kinetochore interactions

Miller, M. P.*, Unal, E.*, Brar, G. A. & Amon, A.
(*) equal contribution

eLife 1, e00117 (2012)

Lactic acid-producing yeast cells having nonfunctional l- or d-lactate:ferricytochrome c oxidoreductase cells

Miller M.P., Suominen P, Aristidou A, Hause B, Van Hoek P, Asleson Dundon C.

United States Patent 8,137,953, Issued March 2012